Atlas of Topographical and Applied Human Anatomy, Vol. 2: by Eduard Pernkopf, Helmut Ferner, Henry Monsen

By Eduard Pernkopf, Helmut Ferner, Henry Monsen

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Additional info for Atlas of Topographical and Applied Human Anatomy, Vol. 2: Thorax, Abdomen and Extremities (v. 2)

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These washing steps are necessary to get rid of penicillin and streptomycin which could be harmful to the PNRC during the following enzymatic digestion. 41. This first digestion allows the removal of broken cells as well as blood cells. The temperature during this digestion step as well as the following ones should be continuously checked and protocoled. It should not exceed 37 °C. Otherwise trypsin-catalyzed digestion will result in excessive cell damage leading to an increased number of dead cells in the sought culture.

9. FCS: Thaw the 500 ml bottle with frozen FCS at room temperature. Inactivate the thawed FCS (if needed) for 30 min at 56 °C using a water bath. Distribute the volume into portions of either 100 ml of 50 ml under sterile conditions and store at −20 °C. 10. Horse serum (HS): Thaw the 500 ml bottle with frozen HS at room temperature. Inactivate the thawed HS (if needed) for 30 min at 56 °C using a water bath. Distribute the volume into portions of either 100 ml of 50 ml under sterile conditions and store at −20 °C.

MicroRNA sponges are antisense inhibitors that competitively bind to their target miRNA [13]. They have been observed to target whole miRNA families through complementarity to seed sequences. These had eight RNA binding sites with perfect complementarity to their target miRNA which allows for specificity [14]. The bulged sponge inhibitor introduces a bulge in the central region of the target site in addition to the eight miRNA recognition sites which decreases endonucleolytic target cleavage, thus improving binding ability to its target mRNA [5].

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